Fig. 9.

Triclocarban (10 μM) inhibited the histone deacetylase (HDAC; a), sirtuin (b), and DNA methyltransferase (DNMT; d) activities but did not affect histone acetyltransferase activity (HAT; c) in neocortical cells. The primary neocortical cultures were treated with triclocarban for 24 h, except for DNMT activity that was measured after 6-h treatment. A positive control contained HeLa cells for HDAC, undefined sample for sirtuins, an active nuclear extract for HAT and MU4 DNMT Enzyme Control for DNMT. A negative control had no cells, and the HDAC inhibitor has been used to inhibit non-sirtuin activities in the samples. The results were normalized to the absorbency in the vehicle-treated cells and expressed as μM, pM, nmol/μg protein, or a percentage of the control. Each bar represents the mean ± SEM. The number of replicates in each experiment ranged from 6 to 8, except for the measurements of sirtuin activity, whose number of replicates was 5. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control cultures