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. 2019 Apr 23;9:6384. doi: 10.1038/s41598-019-42716-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Titer of viable cells present in 24 h DSM cultures of each sporeformer fish isolate (codes in x axis) before (grey, total cells) and after (blue, sporulating or heat resistant cells) a 20 min heat treatment at 80 °C. Sporulation was induced by nutrient exhaustion in liquid Difco Sporulation Medium (DSM) at 37 °C, 150 rpm. Numbers on top of the panel correspond to the percentages (%) of sporulation calculated as the ratio between sporulating cells and total cells. Bacillus subtilis 168 was used as control and the results are the average of three independent experiments with error bars representing the standard deviation.