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. 2019 Apr 23;9:6374. doi: 10.1038/s41598-019-42932-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CD4⁺ T and Th17 cell counts in the spleen were lowest after the combined treatment with rapamycin and DON. (A) The frequency of CD4⁺ T, Th17 (Rorγt⁺/CD4⁺), and Treg (Foxp3⁺/CD4⁺) cells in splenocytes treated with various drug regimens was analyzed by flow cytometry, and the number of CD4⁺ T cells was calculated. (B) The frequency of DCs (CD11c⁺ CD11b⁺), macrophages (F4/80⁺ CD11b⁺), and MDSCs (Gr1⁺ CD11b⁺) in the spleen was analyzed by flow cytometry. (C) The frequency of M-MDSCs (CD11b⁺ Ly6G⁻ Ly6C^high) and G-MDSCs (CD11b⁺ Ly6G⁺ Ly6C^int) was analyzed by flow cytometry. Bars show mean ± SEM; n = 5 rapamycin (Rapa), 6 DON, and 6 Rapa + DON. ^*P < 0.05; ^***P < 0.001; n.s. = not significant.