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. 2019 Apr 16;10:796. doi: 10.3389/fmicb.2019.00796

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Copyright © 2019 Sun, Yue, Yang, Hao, Song, Li, Chen, Chu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biomass and phytase expression of recombinant B. longum JCM 1217 grown in modified reinforced clostridia medium. (A) biomass of different strains assayed by reading optical density at 600 nm after anaerobic incubation for 48 h; (B) phytase activity in the CFMs after 48 h incubation of different strains; (C) phytase activity in the cell lysate after 48 h incubation of different strains; (D) time course phytase activity in CFMs of recombinant B. longum JCM 1217 harboring plasmid pBPES or pBPES-tu. Samples were collected at different time points during fermentation in the modified RCM. CFM, cell free medium; RCM, reinforced clostridia medium.