Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant features or description | Sources |
|---|---|---|
| E. coli DH5a | Type strain | Invitrogen |
| B. longum JCM 1217 | Sequenced wide-type strain | GIMCC |
| B. longum JCM 1217/pBPES | JCM 1217 possessing plasmid pBPES | This study |
| B. longum JCM 1217/pBPES-tu | JCM 1217 possessing plasmid pBPES-tu | This study |
| B. longum JCM 1217/pBPES-groEL | JCM 1217 possessing plasmid pBPES-groEL | This study |
| B. longum JCM 1217/pBPES-gap | JCM 1217 possessing plasmid pBPES-gap | This study |
| pBPES | Escherichia coli-Bifidobacterium shuttle vector, no promoter, bearing signal peptide and appA gene | This study |
| pBPES-tu | pBPES containing 172 bp promoter region of gene elongation factor Tu (BLLJ_0515) | This study |
| pBPES-groEL | pBPES containing 241 bp promoter region of gene groEL (BLLJ_1448) | This study |
| pBPES-gap | pBPES containing 240 bp promoter region of gene GAPDH (BLLJ_1241) | This study |
GIMCC; Guangdong Institute of Microbiology Culture Center, Guangdong Province, China.