Table 2.
Primers used in this study.
| Primer | Nucleotide sequence | Amplicon | Size (bp) |
|---|---|---|---|
| SPR | ATCCGCTGCGTTGGCCGTG | SP | 99 |
| SPF | ATGAAATCACTGATGAAAAAGG | ||
| PhyR | TTACAAACTGCACGCCGGT | mature appA | 1233 |
| PhyF | CAGAGTGAGCCGGAGCTGA | ||
| Soe1R | GGAATTCCATATGTTACAAACTG CACGCCGGT | SP-appA | 1353 |
| Soe1F | CGGAATTCATGAAATCACTGAT GAAAAAGG | ||
| Soe2R | CCCAAGCTTTCAAACGCGCGA GAAACGGCATT | Cmr -pMB1 | 1669 |
| Soe2F | CGAATATTATGGAGAAAAAAATC ACTGGATATAC | ||
| tuR | CGGAATTCTACTTTTGTCCTCCT GGACGTCTC | Ptu | 172 |
| tuF | CCCAAGCTTCACGCGCCACTG CATGAAG | ||
| gapR | CGGAATTCGTAATTCTCCCTTGT AGGGTGG | Pgap | 240 |
| gapF | CCCAAGCTTCTTGCATGCCGC GCGCTTG | ||
| groR | CGGAATTCGAGTAACACGCAC GCAAGGATG | PgroEL | 241 |
| groF | CCCAAGCTTATGGTTGCGTATT CCTCCAAATGGCT | ||
| rPR | ATCCGCTGCGTTGGCCGTG | promoter- | variable |
| SP-appA | |||
| rPF | TCCGGAGACGTCAGCTGCT |
Underlined: digestion sites for restriction enzymes.