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. 2019 Apr 16;6:49. doi: 10.3389/fcvm.2019.00049

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Copyright © 2019 Goto, Rogers, Blaser, Higashi, Lee, Schlotter, Body, Aikawa, Singh and Aikawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Senescence-associated mRNA levels in human VICs were not altered by OM. mRNA levels of tumor promoting protein p53 (TP53), cyclin dependent kinase inhibitor 1A (CDKN1A), and cyclin dependent kinase inhibitor 2A (CDKN2A) in calcific aortic valve disease tissue-derived VICs cultured in control normal media (NM) or OM. n = 3 donors, P1–4 = passage 1–4, ^*P < 0.05, ^***P < 0.001 analyzed by ANOVA with Bonferroni's multiple comparison test, error bars indicate SD.