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. 2019 Apr 16;10:400. doi: 10.3389/fphar.2019.00400

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Copyright © 2019 Sachs, Döhl, Weber, Bonus, Ehlers, Fleischer, Klinger, Gohlke, Pietruszka, Schmitt and Teusch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the transport IC₅₀ values for (A) compound (R)-19, (B) compound (S)-19, (C) compound 16 and (D) compound 16a. For each compound a serial dilution was used to determine the IC₅₀ curve. Membranes were incubated with the compound and active transport was detected with the Tecan Infinite M200.