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. 2019 Apr 16;13:360. doi: 10.3389/fnins.2019.00360

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Copyright © 2019 Márquez-Valadez, Aquino-Miranda, Quintero-Romero, Papacostas-Quintanilla, Bueno-Nava, López-Rubalcava, Díaz, Arias-Montaño and Molina-Hernández.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative analysis of the effect of chlorpheniramine administration to pregnant rats on dopaminergic commitment and differentiation in the ventral mesencephalon of 14- and 16-day-old embryos. Left columns show representative Western blots for LMX1b (green, 41 kDa) in panel (A) and PITX3 (green, 32 kDa) in panel (B), for E14 and E16 ventral mesencephalon (vMes) from embryos from control (Ctl) and chlorpheniramine-treated (Chlo) rats. The internal control (GAPDH, 37 kDa) appears in red. Protein extracts from vMes E12 or adult substantia nigra were used as positive controls (+Ctl) for LMX1b and PITX3, respectively. MW, molecular weight ladder in kDa. Right columns, semi-quantitative fluorometry analysis of LMX1b (A) and PITX3 (B), for E14 and E16 tissues from the vMes. Values are expressed as percentage of the fluorescence ratio of the control tissues and are means ± SEM from four experiments. P-values were obtained after two-tailed unpaired Student’s t-test; n.s., non-significant.