Table 1.
Primer pairs designed for the mutliplex PCR for bacteriocin cluster typing of S. dentisani.
| Primer | Target | Sequence | Amplicon size | Ta [°C] | Primer Mix |
|---|---|---|---|---|---|
| S.d-Blp_F S.d-Blp_R |
Bacteriocin BlpN/K of class IIb | CTTGCGTTGAAGGTGGAGAT GCTCCCCAAGCTGCTGTA |
160 | 57 | 1 |
| S.d-Pnc_F S.d-Pnc_R |
Bacteriocin PncT of class IIb | GACAATTAGTGGTGGAGATGACTG GGAACTGGAGGTGCAGGAT |
153 | 56 | 2 |
| S.d-Arg.syn_F S.d-Arg.syn_R |
Argininosuccinat synthase | CGCTGTTTGTATGGATGTGG ATTTCCGAGCCACTACCTGA |
968 | 56 | 1 |
| S.d-Arg.lyase F S.d-Arg.lyase R |
Argininosuccinat lyase | AAGAGGCAGAGCAGATCCAA TCAAGGGATGGTAGGTTTGC |
1,089 | 56 | 2 |
| S.d-Lan-effpr F S.d-Lan-effpr R |
Lantibiotic efflux protein | ACGCTCTGACGTTTCTGACA CTCCCATTGACTCCTCAGGT |
534 | 56 | 1 |
| S.d-Mu-lik-pr F S.d-Mu-lik-pr R |
Transcriptional regulator | CTTCGACGAGTTTGGACACA ACATCGTATGGACGGAGCAT |
510 | 56 | 2 |
| Lac1- F Lac1 R |
Lactococcin1 | AAATTGGTGTCGCAACCGTA TCTAATCCTGTTGGCGGAAT |
250 | 58 | 1 |
| Lac2- F Lac2 R |
Lactococcin1 | GTGGTYTGCTTGCTACGACA TCCTGTTGGCGGAATTTTAG |
218 | 56 | 2 |