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. 2019 Apr 16;10:228. doi: 10.3389/fendo.2019.00228

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Copyright © 2019 Liu, Wang, Qin, Xu, Zhou, Xu, Liu, Ye, Yue, Zheng and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of AG on osteoblastic differentiation and matrix mineralization in mouse MC3T3-E1 cells. (A) Viability of MC3T3-E1 cells was measured by MTT assay after 48 h of AG treatment. ^*P < 0.05, ^**P < 0.01 compared with the control group. N = 4. (B) ALP activity was determined by using the Alkaline Phosphatase Assay Kit after 7 and 14 days of AG treatment. ^*P < 0.05 compared with the corresponding blank group. ^#P < 0.05 compared with the corresponding control group. N = 4. (C) Representative images of mineralized nodules after Alizarin Red S staining. Scale bar, 1.0 mm. (D) Quantitative analysis of mineralized nodules after Alizarin Red S staining. ^*P < 0.05 compared with the corresponding blank group. ^#P < 0.05, ^##P < 0.01 compared with the corresponding control group. N = 4. All of the data are shown as the mean ± S.D. of the independent experiments.