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. 2019 Apr 16;10:228. doi: 10.3389/fendo.2019.00228

Figure 4.

Figure 4

The effects of AG on the protein levels of MAPK signaling molecules in MC3T3-E1 cells. MC3T3-E1 cells were treated with AG at 5, 10, and 20 μM for 24 h. (A) The protein levels of p-Erk1/2, Erk1/2, p-JNK, JNK, p-p38, and p38 were measured by western blot. (B) Quantitative analysis of the protein levels of p-Erk1/2 and Erk1/2 in AG-treated MC3T3-E1 cells. (C) Quantitative analysis of the protein levels of p-p38 and p38 in AG-treated mouse MC3T3-E1 cells. (D) Quantitative analysis of the protein levels of p-JNK and JNK in AG-treated mouse MC3T3-E1 cells. (E) Quantitative analysis of the ratios of the phosphorylated proteins to the total proteins in AG-treated mouse MC3T3-E1 cells. All of the data are shown as the mean ± S.D. of the independent experiments. N = 3. *P < 0.05, **P < 0.01 compared with the corresponding blank group. #P < 0.05, ##P < 0.01 compared with the corresponding control group.