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. 2019 Feb 1;8(5):490–499. doi: 10.1002/sctm.18-0162

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© 2019 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Characterization of extracellular vesicles (EVs) isolated from stem cells from the dental pulp of human exfoliated deciduous teeth (SHEDs). (A): Transmission electron microscopy of EVs isolated from SHEDs (×30,000 magnification). A magnified image of EV is shown on the left panel (×120,000 magnification). (B): Determination of the concentration and particle size of EVs derived from SHEDs. Nanoparticle tracking analysis was performed with NanoSight LM10 instrument (Malvern Panalytical). Size distribution of the EVs was around 100 nm. (C): Samples from supernatants (S), cell lysates (L), and EV fractions (EVs) were subjected to electrophoresis, blotted and the membrane was probed with antibodies against EV markers (HSP70, MFG‐E8, syntenin‐1), or LGR5 as a negative control. Bands were visualized by incubation with appropriate horseradish peroxidase‐conjugated secondary antibodies and chemiluminescence substrate.