Table 1.

Primers used for PCR and sequencing of MC1R, ASIP, and TYRP1.

Gene symbol	Region	Forward 5′ – 3′	Reverse 5′ – 3′	Product
and primer ID				size, bp
MC1R 5′ UTR 1	5′ UTR	TCTCACGCCCTTTGAAGTCT	GCTGACGACAAACCCTTCTC	2007
MC1R 5′ UTR 2	5′ UTR	ACACACCTTAACGGGACACC	GCAGGAAAGGGTCTTCACTCT	1101
MC1R 1	Exon 1	TCCTCCTCTGTCTCGTCAGC	GTTGGCTGGCACTGTCTCC	918
MC1R 2	Exon 1	GGCGCTGTCTCTTGTGGAG	GACCAGAAGAGACGCAGGAG	912
MC1R 3	Exon 1	CTCCCTGGCAGGACGATG	AGTCCGAGGTGGGTGGTG	92
MC1R_OV∗	Exon 1	CATGGTGTCCAGCCTCTGCTCTCT	CACGGCGATAGCACCCAGAGAGCA	n/a
ASIP Prom	Promoter	GGATTTGGGGTCAGTCTGTA	CCCATCCCTTTAGCCTCCTA	2608
ASIP Ex1	Exon 1	GTGTGAGTCAGTGGCAGGAA	AAATTCTGGGTGGGCTAAGG	1130
ASIP Ex1b	Exon 1	ACTTAAGGCAGGCTGGACCT	ATGTGCCCATCCCTTTAGC	1502
ASIPex1seq	Exon 1	AGACCCTGCATTAAGCTGCTC	Sequencing primer	n/a
ASIPex1seqb	Exon 1	GCTTTTCTGATAATGAAATA	Sequencing primer	n/a
ASIP Ex 2	Exon 2	CTTCAGTCTCCCTCCCTTCC	GCCAGGTATTTTTCCCTGAG	828
ASIP Ex 3	Exon 3	TCCAGGGCCTTATTGGACTT	CTGGAAAGGCTCAGTTTGCT	953
ASIP Ex 4	Exon 4	ACTGTAAGAGGGCCAGAGCA	TAAAGTAGGGGGCAGCATTG	511
TYRP1 Ex 1	Exon 1	AGCACTTTGAAGGTGGGTTG	AGTCAGAAGACTGGAGCATCAA	698
TYRP1 Ex 2	Exon 2	AAGAGAGGGAGTGGAAGGGAGA	ATGTGAAATTGCTTGGTCAGTG	650
TYRP1 Ex 3	Exon 3	TGAGTTGGGTTTCATTCCTT	CACTTTCTTTTTCCCCTGGA	629
TYRP1 Ex 4	Exon 4	TGGACATGGTAACTTGGGTTT	GGCCAGCAACCTAACTTTGA	722
TYRP1 Ex 5	Exon 5	GGCCACCAACCATAGGTACA	GACTTCCTGTCTGCCTTTTCA	525
TYRP1 Ex 6	Exon 6	CCTGGGCTGCTGTAGTGAA	CTGGGGGCTCTCAACAAACT	500
TYRP1 Ex 7	Exon 7	GGAATTAGGAAGTGCCCTGA	AACATGCCCCAAATCTTCAC	595

Asterisk denotes overgo primers for screening MC1R from CHORI-246 BAC library.