Table 1.
Physiological parameters for mice at the conclusion of the 10 week study (18 weeks of age) showing non-diabetic (control) normotensive mice are heavier than hypertensive animals.
| Variable | Cont. Normot. | Cont. Hypert. | Diab. Normot. | Diab. Hypert. | p-value: Interaction | p-value: Effect of Diabetes | p-value: Difference Between Strains |
|---|---|---|---|---|---|---|---|
| Body Weight (g) | 28 ± 0.6 | 23 ± 0.3* | 24 ± 0.4* | 22 ± 0.5 | <0.001 | <0.001 | <0.001 |
| HbA1c (mmol/mol)(%) | 21 ± 1 (4.1 ± 0.1) | 28 ± 1 (4.7 ± 0.1) | 85 ± 5 (9.9 ± 0.4) ∗ | 111 ± 4 (12.3 ± 0.4) †‡ | <0.001 | <0.001 | <0.001 |
| Plasma glucose (mmol/L) | 8.0 ± 0.8 | 7.7 ± 0.5 | 25.5 ± 0.9* | 23.2 ± 1.1 † | 0.263 | <0.001 | 0.144 |
| Total Cholesterol (mmol/L) | 2.8 ± 0.2 | 2.4 ± 0.2 | 3.5 ± 0.2 | 3.1 ± 0.2 | 0.815 | 0.001 | 0.049 |
| 24 h Water intake (ml) | 3.8 ± 0.5 | 5.2 ± 0.5 | 15.3 ± 1.4* | 18.0 ± 2.0 † | 0.537 | <0.001 | 0.078 |
| % 24 h Water intake/BW (ml/0.01 g) | 13.6 ± 2.0 | 21.8 ± 2.0 | 67.7 ± 6.8* | 83.8 ± 11.1 † | 0.498 | <0.001 | 0.042 |
| 24 h Urine output (ml) | 0.4 ± 0.3+ | 0.3 ± 0.1 | 11.0 ± 1.1* | 11.5 ± 1.0 † | 0.093 | <0.001 | 0.748 |
| % 24 h Urine output/BW (ml/0.01 g) | 1.67 ± 1.14 | 1.32 ± 0.23 | 48.4 ± 5.0* | 53.7 ± 6.1 † | 0.442 | <0.001 | 0.501 |
| 24 h Food consumption (g) | 2.2 ± 0.4 | 3.4 ± 0.3 | 4.9 ± 0.2* | 5.3 ± 0.1 † | 0.287 | <0.001 | 0.025 |
| 24 h Food consumption/BW (g/g) | 0.08 ± 0.02 | 0.14 ± 0.02 | 0.22 ± 0.01* | 0.25 ± 0.01 † | 0.294 | <0.001 | 0.005 |
| Heart weight (wet; mg) | 152.2 ± 7.7 | 149.5 ± 10.0 | 132.7 ± 17.4 | 156.2 ± 16.2 | 0.301 | 0.619 | 0.282 |
| Heart weight/BW (mg/g) | 5.3 ± 0.2 | 6.3 ± 0.3 | 5.9 ± 0.8 | 7.5 ± 0.8 | 0.519 | 0.166 | 0.024 |
| Left kidney weight (wet; mg) | 234.8 ± 5.9 | 186.1 ± 4.9* | 267.2 ± 4.7* | 265.5 ± 12.8 † | 0.005 | <0.001 | 0.001 |
| Left kidney weight/BW (mg/g) | 8.2 ± 0.1 | 7.9 ± 0.1 | 11.8 ± 0.3* | 12.6 ± 0.5 † | 0.036 | <0.001 | 0.146 |
| Right kidney weight (wet;mg) | 249.8 ± 8.6 | 197.1 ± 5.1* | 290.9 ± 8.7* | 269.6 ± 16.6 † | 0.015 | <0.001 | 0.018 |
| Right kidney weight/BW (mg/g) | 8.8 ± 0.2 | 8.3 ± 0.2 | 12.9 ± 0.6* | 12.8 ± 0.2 † | 0.427 | <0.001 | 0.121 |
As expected diabetic strains had elevations in plasma glucose and total cholesterol with no difference between strains, diabetic hypertensive animals had a slightly higher HbA1c. Data represents mean ± SEM. Body weight (BW) n = 21–29; organ weights n = 6–10, 24 h metabolic caging data n = 7–10; HbA1c n = 12–18, plasma cholesterol and glucose n = 13–17; plasma triglycerides 6–9. Cont., non-diabetic Control; Diab., diabetic; Normot., normotensive BPN/3J Schlager mice; Hypert., hypertensive BPH/2J Schlager mice. +, please note for urine volume only 4 Cont. Normot. animals produced urine. Significant p-values found with 2-factor ANOVA are in bold. t-test p < 0.05: ∗ vs. Cont. Normot.; † vs. Cont. Hypert.; ‡ vs. Diab. Normot.