Table 2.
Gene expression for markers of fibrosis, components of the renin-angiotensin system, the catecholaminergic system and pro- and anti-oxidant systems in renal cortex of mice.
| Gene | Cont. Normot. | Cont. Hypert. | Diab. Normot. | Diab. Hypert. | p-value: Interaction | p-value: Effect of diabetes | p-value: difference between strains |
|---|---|---|---|---|---|---|---|
| Fibrotic | |||||||
| acta2 (α2 smooth muscle actin) | 1.0 ± 0.1 | 1.7 ± 0.3 | 1.3 ± 0.3 | 2.0 ± 0.4 | 0.921 | 0.208 | 0.023 |
| fn1 (fibronectin) | 1.0 ± 0.1 | 0.9 ± 0.2 | 2.0 ± 0.4 | 2.4 ± 0.5 ‡ | 0.485 | 0.002 | 0.719 |
| col4a1 (collagen IV) | 1.0 ± 0.2 | 1.6 ± 0.4 | 1.3 ± 0.4 | 2.1 ± 0.4 | 0.729 | 0.302 | 0.043 |
| ctgf | 1.0 ± 0.2 | 2.5 ± 0.7 | 1.2 ± 0.3 | 4.3 ± 1.5 | 0.429 | 0.334 | 0.028 |
| vegfa (VEGF) | 1.0 ± 0.2 | 1.5 ± 0.2 | 0.8 ± 0.2 | 1.4 ± 0.1 | 0.997 | 0.353 | 0.004 |
| rela (p65 subunit of NFκB) | 1.0 ± 0.2 | 2.4 ± 0.4 | 1.5 ± 0.5 | 3.4 ± 0.6 | 0.559 | 0.141 | 0.003 |
| Renin-Angiotensin System | |||||||
| ren1 (renin) | 1.0 ± 0.1 | 1.0 ± 0.3 | 1.1 ± 0.2 | 0.7 ± 0.1 | 0.311 | 0.690 | 0.365 |
| ace | 1.0 ± 0.1 | 1.6 ± 0.2* | 0.5 ± 0.1 | 0.6 ± 0.1 † | 0.187 | <0.001 | 0.060 |
| agtr1(AT1A receptor) | 1.0 ± 0.2 | 1.7 ± 0.3 | 1.5 ± 0.4 | 1.0 ± 0.3 | 0.678 | 0.723 | 0.051 |
| agtr2 (AT2 receptor) | 1.0 ± 0.2 | 3.8 ± 1.1* | 1.5 ± 0.2 | 4.8 ± 0.7 ‡ | 0.718 | 0.234 | < 0.001 |
| Catecholaminergic | |||||||
| dbh | 1.0 ± 0.2 | 1.9 ± 0.3 | 1.4 ± 0.4 | 1.8 ± 0.5 | 0.507 | 0.778 | 0.088 |
| adra1b (α adrenoceptor 1b subunit) | 1.0 ± 0.1 | 1.3 ± 0.2 | 1.7 ± 0.2 | 2.4 ± 0.3 | 0.998 | 0.013 | 0.272 |
| adrb1 (β1 adrenoceptor) | 1.0 ± 0.1 | 0.7 ± 0.1 | 1.3 ± 0.3 | 0.8 ± 0.1 | 0.383 | 0.281 | 0.008 |
| slc6a2 (norepinephrine transporter) | 1.0 ± 0.1 | 0.7 ± 0.2 | 2.1 ± 0.3* | 2.1 ± 0.3 | 0.229 | 0.003 | 0.171 |
| comt | 1.0 ± 0.1 | 1.2 ± 0.2 | 1.6 ± 0.4 | 1.5 ± 0.2 | 0.424 | 0.069 | 0.868 |
| maoa | 1.0 ± 0.2 | 1.0 ± 0.4 | 2.0 ± 0.4 | 3.8 ± 0.9 † | 0.131 | 0.003 | 0.118 |
| maob | 1.0 ± 0.2 | 0.7 ± 0.1 | 1.4 ± 0.5 | 0.9 ± 0.1 | 0.624 | 0.191 | 0.054 |
| Pro-oxidant | |||||||
| nox1 | 1.0 ± 0.2 | 1.6 ± 0.4 | 3.1 ± 1.1 | 3.0 ± 0.8 | 0.561 | 0.005 | 0.675 |
| cybb (Nox2) | 1.0 ± 0.1 | 0.8 ± 0.2 | 1.4 ± 0.4 | 1.0 ± 0.2 | 0.674 | 0.152 | 0.165 |
| nox4 | 1.0 ± 0.1 | 2.7 ± 0.8 | 0.9 ± 0.2 | 2.2 ± 0.4 | 0.691 | 0.548 | 0.005 |
| ncf1 (p47phox) | 1.0 ± 0.1 | 0.7 ± 0.1 | 1.1 ± 0.2 | 1.3 ± 0.2 † | 0.084 | 0.010 | 0.523 |
| Anti-oxidant | |||||||
| sod1 (CuZnSOD; SOD1) | 1.0 ± 0.1 | 1.1 ± 0.1 | 0.9 ± 0.2 | 1.1 ± 0.1 | 0.948 | 0.732 | 0.373 |
| sod2 (MnSOD; SOD2) | 1.0 ± 0.2 | 1.0 ± 0.2 | 0.7 ± 0.1 | 1.2 ± 0.1 | 0.119 | 0.707 | 0.086 |
| cat (catalase) | 1.0 ± 0.1 | 1.5 ± 0.2* | 0.9 ± 0.1 | 1.5 ± 0.1 ‡ | 0.797 | 0.507 | 0.001 |
The protein encoded by the gene is in brackets. n = 3–9. Data presented as mean ± SEM. Significant p-values found with 2-factor ANOVA are in bold. t-test p ≤ 0.05: ∗ vs. Cont. Normot.; † vs. Cont. Hypert.; ‡ vs. Diab. Normot.; Note- rela Diab. Normot. vs. Diab. Hypert. p = 0.057, nox4 Cont. Normot. vs. Cont. Hypert. p = 0.066.