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. 2019 Apr 16;10:386. doi: 10.3389/fphys.2019.00386

FIGURE 4.

FIGURE 4

Whole-cell recordings of ion currents from RBCs of healthy donors and HS patients. Whole-cell recordings of ion currents from RBCs of healthy donors and HS patients with ankyrin mutations (A–C). Compared are the I/V-curves of all HS patients with ankyrin mutations (N = 6) with the I/V curves of their own transportation controls (N = 4) (Aa) and with the I/V curves of all healthy subjects delivered throughout the study (N = 27) (Ab), where N denotes the number of healthy subjects or HS patients. No changes were observed in capacitance either with the transportation (0.67 pA/pF patients vs. 0.63 pA/pF transportation control; p > 0.05) or with the general control (0.67 pA/pF patients vs. 0.69 pA/pF general control; p > 0.05). Compared are the I/V curves of P17.1 (n = 13) and its own transportation control C17 (n = 6) (Ba) and the I/V curves of P17.2 (n = 11) with its own transportation control C17 (n = 6) (Ca), where n denotes the number of cells from the patient or the control. (Bb,Cb) Compare the I/V curve of a general control based on currents recorded from all cells of all healthy subjects delivered throughout the study (n = 175) with the I/V curve of P17.1 (n = 13) (Bb) and P17.2 (n = 11) (Cb) (n denotes number of cells). Both patients 17.1 and 17.2 showed no differences in capacitance either with their transportation or with the general control (0.67 pA/pF P17.1 vs. 0.525 pA/pF C17, p > 0.05; 0.67 pA/pF P17.1 vs. 0.69 pA/pF general control, p > 0.05; 0.62 pA/pF P17.2 vs. 0.525 pA/pF C17, p > 0.05; 0.62 pA/pF P17.2 vs. 0.69 pA/pF general control, p > 0.05). However, a difference was uncovered between C17 and the general control (0.525 pA/pF C17 vs. 0.69 pA/pF general control; p < 0.05). Whole-cell recordings of ion currents from RBCs of a healthy donor and a HS patient with band 3 protein mutation (D). Compared are the I/V curves of P12.1 (n = 17) with its own transportation control C12 (n = 7) (Da) as well as with a general control (n = 175) (Db), where n is the number of cells of the patient or the controls. No changes were observed in capacitance either with the transportation (0.65 pA/pF P12.1 vs. 0.59 pA/pF C12; p > 0.05) or with the general control (0.65 pA/pF P12.1 vs. 0.69 pA/pF general control; p > 0.05). Currents were elicited by voltage steps from –100 to 100 mV for 500 ms in 20 mV increments at Vh = –30 mV. Data are expressed as mean current density ± SEMs. Significant differences are determined based on an unpaired t-test with ∗ representing p < 0.05. Mutations below patients numbers are designated as amino acid substitutions in the respective protein.