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. 2019 Apr 16;10:754. doi: 10.3389/fmicb.2019.00754

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Copyright © 2019 Shifman, Steinberger-Levy, Aloni-Grinstein, Gur, Aftalion, Ron, Mamroud, Ber and Rotem.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dose-dependent expression of Yersinia pestis genes upon exposure to doxycycline. Y. pestis Kim53 bacteria were exposed for 2 h to the indicated concentrations of doxycycline. After 2 h, the bacteria were harvested and subjected to transcriptomic analysis using a custom Agilent DNA microarray. (A) The number of genes that were upregulated (blue circle) or downregulated (orange square) by at least threefold upon exposure to doxycycline. (B) The maximal change in expression levels that was observed at each doxycycline concentration in the upregulated (blue circle) or downregulated genes (orange square).