. 2019 Apr 16;10:754. doi: 10.3389/fmicb.2019.00754

Table 1.

The change in the candidate gene expression after 2 h of exposure to doxycycline: transcriptomic results.

				Fold change^a

				Doxycycline (μg/ml)

	0.125	0.25	0.5	1	2	4	8	MIC FC ratio
Gene name (ORF)^b	0.5× MIC	1× MIC^c	2× MIC	4× MIC	8× MIC	16× MIC	32× MIC	$\frac{1 \times MIC}{0.5 \times MIC}$	$\frac{32 \times MIC}{0.5 \times MIC}$
lcrF (YPCD1.49)	2.2	7.2	14.6	28.8	29.7	23.0	27.5	3.2	12.3
yscA (YPCD1.50)	4.3	15.7	35.3	56.1	56.0	41.2	29.2	3.6	6.8
ORFC (YPCD1.65)	2.0	5.1	6.9	12.4	14.5	12.5	10.3	2.6	5.2
mgtB (YPO1661)	3.5	10.5	19.6	36.2	44.8	48.3	47.9	3.0	13.8
iucA (YPO0989)	−1.4	−3.6	−8.6	−15.3	−17.4	−13.0	−14.5	2.5	10.2
bioD (YPO1154)	−1.3	−3.4	−7.2	−9.7	−10.5	−11.5	−12.4	2.6	9.4
fyuA (YPO1906)	−2.3	−7.2	−14.7	−22.6	−24.6	−29.7	−26.4	3.2	11.5
irp5 (YPO1907)	−2.9	−9.2	−14.0	−20.5	−23.5	−30.0	−30.1	3.2	10.3
irp4 (YPO1908)	−3.3	−11.9	−18.5	−35.1	−39.9	−39.5	−45.8	3.6	13.9
irp3 (YPO1909)	−3.9	−12.2	−18.2	−27.0	−31.6	−34.0	−36.4	3.1	9.3
irp1 (YPO1910)	−3.7	−12.0	−16.0	−25.5	−28.0	−28.8	−35.1	3.3	9.6
irp2 (YPO1911)	−2.8	−11.2	−21.5	−37.4	−41.2	−55.1	−56.2	4.0	20.0
irp6 (YPO1913)	−2.9	−9.6	−19.7	−35.4	−44.9	−55.4	−55.8	3.4	19.5
irp7 (YPO1914)	−3.0	−10.8	−21.0	−40.6	−56.1	−56.9	−64.6	3.6	21.7
irp9 (YPO1916)	−2.0	−7.6	−15.6	−21.9	−26.5	−30.7	−31.4	3.7	15.4

^aThe change in the gene expression levels was determined for the Y. pestis Kim53 strain exposed for 2 h to the indicated doxycycline concentrations versus the expression in unexposed control bacteria. Negative FCs represent downregulated responses. The red and green intensity gradients are according to the FC values. ^bGene names and ORFs are based on the annotation of Y. pestis CO92 chromosome and plasmids sequences (Accession Nos. AL590842, AL117211, AL117189, and AE017046). ^cThe 1× MIC value (0.25 μg/ml doxycycline) was determined in a parallel standard microdilution assay.