Figure 1.

Replication of HuNoVs in IECs derived from human iPSCs. Monolayered human iPSC–derived IECs were inoculated with 2 × 10⁶ genome equivalents of the indicated HuNoV genotypes. Inoculation and sampling were done as described in the Materials and Methods. Viral genome RNA was extracted from both supernatants (ie, those taken at 3 and 72 hours postinfection [hpi]), and then genome equivalents were quantified with reverse transcriptase quantitative polymerase chain reaction. Samples at 3 hpi were used as references. Each value is representative of at least 3 independent experiments and is shown as the mean ± SD from between 4 and 6 wells of supernatants of each culture group.