Supplementary Figure 1.

(A) Weight of WT and CSf1^op/op mice (P = NS; Mann–Whitney test; N = 7 mice for each group). (B and C) Major histocompatibility complex class II (MHCII) macrophages (green) and Protein gene product 9.5 (PGP 9.5) fibers in smooth muscular layers (upper panels) and myenteric plexus (lower panels). The small panels show orthogonal views generated by projecting the z-series in the x (right) and on the y plane (above). Arrows point to macrophage/fiber interactions and squares show macrophage/fiber interactions in orthogonal views. PGP 9.5 immunoreactivity was unusually bright in the cell bodies of myenteric neurons in CSf1^op/op mice when compared with WT tissues. Scale bars: (B) 20 μm, (C) 10 μm.