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. 2019 Apr 16;10:370. doi: 10.3389/fphar.2019.00370

Figure 1.

Figure 1

Inhibition of Notch by DAPT reduces breast cancer migration by non-canonical Notch pathway in 12 h. (A) The expression of NICD was detected in MDA-MB-468 and MDA-MB-231 cells when the cells were treated with 0–50 μM DAPT. (B) Images were taken at 0 and 12th h after wounding by an inverted microscope. (C) Relative migration rate was calculated and the migration of DAPT untreated cells at 12 h was set as 100%. *P < 0.05 DAPT treated cells at 12 h vs. DAPT untreated cells at 12 h. (D) Transwell assay was performed on control vs. 20 μM DAPT treated cells to elevate the migration of cells. Migration index of Ctrl cells at 12 h was set at 100%. *P < 0.05 DAPT-treated cells vs. Ctrl cells. (E) The effect of DAPT on the expression of Hes1 mRNA at indicated time. *P < 0.05 DAPT-treated time point vs. DAPT treated 0 h. (F) The effect of siRBPJκ on the expression of RBPJκ mRNA. *P < 0.05 cells transfected with siRBPJκ vs. cells transfected with siCtrl. (G) The effect of DAPT on the migration of cells transfected with siCtrl or siRBPJκ. *P < 0.05 DAPT-treated cells vs. DAPT-untreated cells.