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. 2019 Apr 16;10:370. doi: 10.3389/fphar.2019.00370

Figure 2.

Figure 2

The migration of breast cancer cells was inhibited by DAPT through Cdc42 pathway. (A,B) The ratio of Cdc42-GTP/Cdc42 and Rac1-GTP/Rac1 were analyzed by Pulldown assay in MDA-MB-468 and MDA-MB-231 cells, which were incubated with DAPT (20 μM) for indicated time. *P < 0.05 DAPT-treated time point vs. DAPT treated 0 h. (C) Effects of Cdc42-Q61L and Cdc42-T17N on the migration of cells. *P < 0.05 cells transfected with plasmid vs. cells transfected with vector. (D,E) Inhibition of Cdc42-T17N or ML141 (Cdc42 inhibitor, 10 μM) on the DAPT-induced migration of MDA-MB-468 and MDA-MB-231 cells. *P < 0.05 DAPT-treated cells vs. DAPT-untreated cells. (F) Analysis of the level of active Cdc42 in breast cancer cells transfected with siRBPJκ (3#) and then incubated with DAPT (20 μM) for indicated time. *P < 0.05 cells transfected with siRBPJκ vs. cells transfected with siCtrl.