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Release of infectious virus by flavivirus-infected human NECs. Apical and basolateral virus shedding up to 72 h p.i. with ZIKV (A,E), JEV (B,F), WNV (C,G), and USUV (D,H) measured by TCID50 assay in NECs infected at a MOI of 1 TCID50/cell. Each symbol represents an independent donor (donor I: dot, donor II: square, donor III: upward triangle, donor IV: diamond, donor V: circle, and donor VI: downward triangle).
