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. 2019 Apr 16;6(2):ENEURO.0451-18.2019. doi: 10.1523/ENEURO.0451-18.2019

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Copyright © 2019 Tuscher et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — hM4Di and eGFP viral expression in the mPFC is present three weeks after injection. Coronal sections (40 µm) of CaMKIIα-hM4Di-mCitrine DREADD (A, C) or CaMKIIα-eGFP control virus (B, D) in the mPFC of female mice three weeks after injection confirms virus expression throughout the mPFC. Blue puncta: DAPI, a marker of cell nuclei; yellow: mCitrine-tagged DREADD virus; green: eGFP-tagged control virus. Merged images provide additional spatial context for DREADD or control virus expression within the region of interest.