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Frequency sweeps of A4H3B4H3-containing hydrogels in PIPPS buffer. (A) Frequency sweeps were performed in the absence of metal ions, in the presence of 1:1 Ca2+:His, 1:1 Zn2+:His and 1:1 Zn2+:His + 25 mM EDTA. The frequency sweeps were performed over a range of 100 to 0.001 rad s−1 at a constant strain amplitude of 1%. (B) Schematic representation of the cross-linking mode in hydrogels without metal ions, with 1:1 Zn2+:His and after adding EDTA.
