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. 2019 Apr 10;24:101192. doi: 10.1016/j.redox.2019.101192

Fig. 3.

Fig. 3

Treatment with NMN rescues age-related downregulation of mitochondrially encoded subunits of the electron transport chain without promoting mitochondrial biogenesis. A-C) Representative electronmicrographs showing mitochondria in cerebromicrovascular endothelial cells in young (A), aged (B) and NMN treated aged mice (C); (arrowheads, mitochondria; nu, nucleus; bm, basal lamina; AC, astrocyte; scale bar: 500 nm). D) Summary data showing that NMN treatment does not affect mitochondrial volume density in aged cerebromicrovascular endothelial cells. E) NMN treatment of aged mice does affect mtDNA content in cerebral arteries. F) NMN treatment (5 × 10−4 moL/L; for 5 days) of aged CMVECs does not affect mtDNA content. G) NMN supplementation rescues age-related decreases in mRNA expression of mitochondrially encoded subunits of the electron transport chain in cerebral arteries. Data are mean ± SEM (n = 5–6 for each data point in D-G). *P < 0.05 vs. Young; #P < 0.05 vs. Aged. (one-way ANOVA with post-hoc Tukey's test). n.s.: not significant.