Table 3.
mStau2 dsRBD1–2 mutant binding to SRS* and SRS2 + 5 RNA
| mStau2 dsRBD1–2 protein | Binding | Kinetics | KD1 [nM] | KD2 [nM] | Hill coefficient |
|---|---|---|---|---|---|
| S RS* RNA | |||||
| wt | Two-site | Transient | 25 ± 8 | n.d. (>1000) | – |
| E15A | Two-site | Transient | 15 ± 11 | 406 ± 359 | – |
| H36A | Hill | Transient | – | 499 ± 149 | 0.9 ± 0.06 |
| F40A | Hill | Transient | – | 769 ± 427 | 1 ± 0.1 |
| K59A | Hill | Transient | – | 624 ± 142 | 0.9 ± 0.2 |
| K60A | Two-site | Transient | 36 ± 5 | n.d. (>1000) | – |
| K59A K60A | Hill | Transient | – | n.d. (>1000) | 1 ± 0.1 |
| E99A | Two-site | Transient | 14 ± 0.7 | 850 ± 295 | – |
| K106A | Two-site | Transient | 30 ± 10 | n.d. (>1000) | – |
| F157A | Hill | Stable | – | n.d. (>1000) | 1.2 ± 0.2 |
| H169A | Hill | Transient | – | n.d. (>1000) | 1 ± 0.6 |
| F40A F157A | No fit | Transient | – | – | – |
| F40A H169A | No fit | Transient | – | – | – |
| SRS2 + 5 RNA | |||||
| wt | Two-site | Transient | 130 ± 30 | n.d. (>1000) | – |
| E15A | Hill | Transient | – | 132 ± 31 | 1 ± 0.1 |
| H36A | Hill | Transient | – | 701 ± 386 | 1 ± 0.2 |
| F40A | Hill | Transient | – | n.d. (>1000) | 1.2 ± 0.1 |
| K59A | Hill | Transient | – | 509 ± 136 | 1.2 ± 0.2 |
| K60A | Hill | Transient | – | n.d. (>1000) | 1 ± 0.1 |
| K59A K60A | Hill | Transient | – | n.d. (>1000) | 1 ± 0.02 |
| E99A | Hill | Transient | – | 635 ± 308 | 1.5 ± 0.3 |
| K106A | No fit | Transient | – | n.d. (>1000) | – |
| F157A | Hill | Stable | – | 497 ± 238 | 1.5 ± 0.2 |
| H169A | No fit | Transient | – | n.d. (>1000) | – |
| F40A F157A | No fit | Stable | – | – | – |
| F40A H169A | No fit | Transient | – | – | – |
± indicates standard deviation