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. Author manuscript; available in PMC: 2019 May 16.
Published in final edited form as: J Am Chem Soc. 2018 May 3;140(19):6137–6145. doi: 10.1021/jacs.8b02461

Figure 5.

Figure 5

Phage display to discover peptide probes for a LOS- strain of A. baumannii (AB5075). (a) Flow cytometry analysis of the representative peptide hit KAM8 in staining A. baumannii (LOS− vs LOS+) in the presence of 1 mg/mL BSA. Replicate data set is presented in the Supporting Information (Figure S9), which shows consistent result. (b) A. baumannii cell staining examined by microscopy with TAMRA labeled KAM8 at 2 µM for LOS− and 10 µM for LOS+. Scale bar: 10 µm. (c) Percent cell killing of A. baumannii (LOS−) with and without photoirradiation. (d) Percent cell killing of the LOS+ versus LOS− strains of A. baumannii with 2 µM KAM8-Eosin. The contrasting outcomes of these strains highlight the high strain specficiy of KAM8.