Table 2.

4-Hydroxyphenylacetic acid production in E. coli DOPA-30N harboring different biosynthetic pathways

Host strain	Plasmid	OD600	Titer (g/L)	Yield (%, mol/mol)
E. coli DOPA-30N	pBbB2k-ipdC-L-feaB	16.28 ± 0.45	2.32 ± 0.05	11.8 ± 0.2
	pBbB2k-ARO10-L-feaB	17.36 ± 0.19	3.08 ± 0.05	13.4 ± 0.2
	pBbB2k-aas-L-feaB	17.13 ± 1.44	2.11 ± 0.06	9.3 ± 0.2
	pBbB2k-tydC-L-tynA-L-feaB	15.44 ± 0.42	2.41 ± 0.04	12.1 ± 0.2
	pBbB2k-ARO10*-L-feaB*	16.68 ± 0.35	5.64 ± 0.06	22.9 ± 0.3
	pBbB2k-ARO10*-TIGR-feaB*	17.12 ± 0.32	6.58 ± 0.16	27.5 ± 0.3

Data represent the means of three replicates and standard deviations