Fig. 6.

The effect of potassium concentrations and TRK1 on the tolerance to other organic acids. WT, trk1Δ and the end-point-evolved isolate (PA-3) were grown to an OD₆₀₀ = 0.2, serially diluted to 1:10, 1:100, 1:1000, and then spotted on YNB agar containing 1 mM and 100 mM potassium, without additional acids and with 83 mM acetic acid, 111 mM lactic acid, 2 mM benzoic acid, and 2 mM sorbic acid. The plates were incubated for 3 days at 30 °C