Fig. 2.

Sequence conservation of MSTN promoter and 5′ regulatory region across species. a Orthologous blocks within ~ 2 kb of MSTN 5′ regulatory region, based on results obtained in multiple combinations of alignments in Mulan [110], reveal evolutionarily conserved blocks mainly among species from the same group (red blocks). Only short regions of MSTN proximal promoter are shared by different vertebrates groups. Among fish mstn-2 promoters, no orthologous blocks were identified when aligning the nine sequences together. Similarly, no conserved segments were found among the three invertebrate species. b Multiple sequence alignment of the MSTN proximal promoter region conserved in fishes, birds and mammals (represented by the asterisks in a), showing the conserved CAAT and TATA boxes (red outlined boxes). Alignment in (b) was obtained and adapted from MAFFT and Pro-Coffee results [111, 112]