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. 2019 Apr 22;12:234. doi: 10.1186/s13104-019-4273-5

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cytokine release from monocytoid THP-1 cells in response to 24 h exposure to MBZ (a) and AZ191 (b). Corresponding cytokine release from PMA differentiated THP-1 macrophages in response to M1 stimuli LPS/IFNγ (c), M2 stimuli IL4/IL13 (d), MBZ 10 µM (e) and AZ191 10 µM (f) after 48 h of incubation (30 h PMA + 18 h with added stimuli and drugs). The results are expressed as relative fluorescence units (RFU) and presented as mean values ± standard deviation for duplicate measurements. Measurements was performed by a Luminex MAGPIX instrument and a multiplex kit from Biorad. The measurements of selected cytokines (IL1β and TNF) were repeated in 4 independent experiments with similar results. See “Results” for details