Fig. 6.

Immunohistochemical staining for C3 in the brains of wild-type and APP/PS1 mice after 12 weeks of exposure to fluoride. WT, wild-type; LF, low fluoride; HF, high fluoride. a WT mice without exposure. b WT receiving LF. c WT receiving HF. d APP/PS1 mice without exposure. e APP/PS1 mice receiving LF. f APP/PS1 receiving HF. Magnification: a–f, × 200, scale bar = 50 μm. g Positive area % of Iba-1 immunostaining detected by immunohistochemistry. The values shown are the means ± SD (n = 10). *P < 0.05 compared to APP/PS1 mice, as determined by analysis of variance (ANOVA), followed by the least significant differences post hoc test