Fig. 11.

Conditioned media from cortical astrocytes infected with MeCP2 adenovirus and conditioned media from cortical astrocytes from male MeCP2-Tg mice kills cortical neurons. a, b Pretreatment with an NMDA receptor antagonist, MK801 (1 μM), prevents astrocyte conditioned media from killing cortical neurons (n = 3). c Glutamate levels are increased in the media of pure astrocyte cultures or mixed neuronal + astrocyte cultures overexpressing MeCP2, but not pure neuronal cultures overexpressing MeCP2. d Tau expression is increased in cortical neurons after treatment with astrocyte conditioned media from astrocytes overexpressing MeCP2 (n = 2). e Tau expression is increased in cortical neurons after treatment with astrocyte conditioned media from male WT or MeCP2-Tg mice (n = 2)