Table 3.

Epigenetic dysregulation in aging: non-coding RNAs.

Regulation	ncRNA	Observed in	Sample size	Approach	Methods	Reference

^≠	miRNA lin-4	C. elegans	N.S.	Targeted	RFLP mapping, Southern Blot, Northern Blot	Lee et al., 1993
^↑	miR-34	C57BL/6J mice	N.S.	Genome-wide	microRNA microarray	Maes et al., 2008
^↑	miR-93
^↑	miR-2l4
^↑	miR-669c
^↑	miR-709
^↓	miR-24	Human (blood)	10-14	Targeted	miRNome miRNA profiling	Noren Hooten
^↓	miR-103			(800 miRNAs)		et al., 2010
^↓	miR-107
^↓	miR-l28
^↓	miR-130a
^↓	miR-155
^↓	miR-221
^↓	miR-496
^↓	miR-1538
^≠	miR-29	C57BL/6J mice and human	Mice: 4; Human: l09	Genome-wide	microRNA microarray	Boon et al., 2011
	miR-34a	Sirt1^+/−, Ku80^−/− and miR-34a^−/− mice	≥4	Targeted (570 miRNAs)	PCR, lSH	Boon et al., 2013
	miR-l46a	HUVEC	-	Genome-wide	microRNA microarray	Vasa-Nicotera et al., 2011
		HUVEC, HAEC, HCAEC	-	Targeted (367 miRNAs)	microRNA microarray, PCR	Olivieri et al., 2013
	miR-217	HUVEC, HAEC, HCAEC	-	Targeted (10 miRNAs)	microRNA microarray, PCR, Northern Blot	Menghini et al., 2009
^↓	miR-17	Human cell lines	-	Targeted (599 miRNAs)	microRNA microarray, PCR	Hackl et al., 2010
^↓	miR-l9c
^↓	miR-20a
^↓	miR-106a
^↓	miR-l46a	HUVEC	-	Genome-wide	microRNA microarray	Vasa-Nicotera et al., 2011
^↑	miR-26a
^↑	miR-181a
^↑	miR-221
^↑	miR-200b
^↑	miR-200c	Human cell lines	-	Targeted (1 miRNA)	WB	Magenta et al., 2011
^↑	miR-144	Human (Cortex and Cerebellum) Chimpanzee Macaque monkey	8 4 5	Genome-wide	microRNA microarray	Persengiev et al., 2011
^≠	miR-l6	SAMP8 mouse	N.S.	Targeted (7 miRNAs)	PCR, ISH, IHC	Liu et al., 2012b

^↑

indicates increased expression levels,

^↓

indicates decreased expression levels, and

^≠

indicates altered expression, not further specified.

Abbreviations: C57BL/6J mice, C57 black 6 inbred mouse strain; C. elegans, Caenorhabditis elegans; miR, micro RNA; SAMP8 mouse, senescence-accelerated prone mouse 8; HUVEC, human umbilical vein endothelial cells; HAEC, human aortic endothelial cells; HCAEC, human coronary artery endothelial cells; N.S., not specified; RFLP, Restriction fragment length polymorphism; PCR, polymerase chain reaction; ISH: in situ hybridization; WB, Western blot; IHC, immunohistochemistry.