Table 1.
Summary of nanodisc stability under different storage conditions.
| Buffer | Temperature (°C) | Days stable/ total days | |||
|---|---|---|---|---|---|
| NDs with POPC | NDs with POPC:POPG (4:1) | ||||
| dH5 | cNW9 | dH5 | cNW9 | ||
| i. 20 mM Tris pH 7.5 50 mM NaCl and 1 mM EDTA | 4 | 30/30 | 30/30 | 1/30 | 30/30 |
| ii. 20 mM PO4 pH6.5 50 mM NaCl and 1 mM EDTA | 4 | 3/30 | 30/30 | 1/30 | 30/30 |
| iii. 20 mM Bis-Tris pH6.5 50 mM NaCl and 1 mM EDTA | 4 | 3/30 | 30/30 | 1/30 | 30/30 |
| 25 | – | – | 1/2 | 2/2 | |
| 37 | – | – | 0/2 | 2/2 | |
| −20 | – | – | 2/2 | 2/2 | |
Circular and linear (cNW9 and dH5) nanodiscs containing either zwitterionic (POPC) or anionic [POPC:POPG (4:1)] lipid mixtures were stored at different temperatures in different buffers (all including 50 mM NaCl and 1 mM EDTA). The stability of the discs was monitored by size-exclusion chromatography using an S200 Increase 5/150 column (GE Healthcare), at daily intervals.