Figure 2.

P. falciparum infected erythrocytes uptake high molecular weight kininogen from the extracellular medium. Schizont-enriched P. falciparum cultures were incubated overnight with 70 μg/mL high molecular weight kininogen conjugated to fluorescein isothiocyanate (HK-AF488) at 37°C. The cells were harvested, plated in poly-lysine-coated microscope dishes, and analyzed by fluorescence microscopy. Images were analyzed using green (internalized HK-AF488) and blue (parasite nucleus) filters or DIC (erythrocytes). RBC, non-infected erythrocytes; Pf-iRBC, infected erythrocytes. Scale bar = 5 μm.