Table 2.
Marker regions used for molecular typing in this study.
| Marker region | Location (gene) | Position in genome relative to Md5 (AF243438) reference | Amplicon size | Putative gene function |
|---|---|---|---|---|
| M1 | Partial UL49.5 and UL50 (C-C) | 112076–112841 | 765 | UL49.5: membrane glycoprotein involved with immune evasion (Tischer et al. 2002). UL50: dUTPase |
| M2 | LORF11 | 124575–125159 | 584 | Associated with viral replication (Lee et al. 2007) |
| M3 | RLORF7 (meq) | 5612–6862 | 1251 | Contributes to MDV oncogenesis by facilitating latency entry/reactivation and by mediating transformation of the target cells (Lupiani et al. 2004) |
| M4 | ICP4 (prox. region) | 144162–145010 | 849 | Immediate-early gene located in the RS regions that is thought to play a role in latency and transformation (Xie, Anderson, and Morgan 1996) |
| M5 | ICP4 (dist. region) | 147700–148181 | 482 | |
| M6 | pp38 | 13841–14665 | 824 | Present in RL. Involved in the establishment of cytolytic infection in B lymphocytes and latency of infected T cells (Gimeno et al. 2005) |
| M7 | UL36 | 88233–88634 | 401 | Large tegument protein (Klupp et al. 2002) |
| M8 | UL43 | 101766–102106 | 340 | Putative tegument protein |