Table 2.
Enzyme | Substrate | Specific activity μmol (min.mg)−1 | Specific activity (with Ca2+ 1mM) μmol (min.mg)−1 |
---|---|---|---|
BoGH9 | MLG | 0.042 ± 0.012 | 0.36 ± 0.05 |
XyG | 0.023 ± 0.003 | 0.05 ± 0.01 | |
HEC | 0.027 ± 0.004 | 0.15 ± 0.05 | |
CMC | 0.021 ± 0.001 | 0.08 ± 0.01 |
Assays conducted in HEPES-NaOH pH 7.0. Recombinant enzymes were incubated at 37 ºC with the different tested substrates.