Table 1.
RNA-seq validation of stromal cell-specific markers and hematopoietic cell-lineage markers based on normalized gene expression levels (FPKM).
| Lineage | Gene | Average LN-LEC | Average D-LEC | Average LN-BEC |
|---|---|---|---|---|
| Endothelial | CD31/Pecam1 | 30662 | 31820 | 98007 |
| Lymphatic endothelial | Pdpn | 18631 | 63207 | 632 |
| Lyve1 | 60105 | 165926 | 1730 | |
| Prox1 | 8005 | 7925 | 228 | |
| Reln | 2994 | 85960 | 78 | |
| Blood endothelial | Vegfr1 (Flt1) | 195 | 61 | 14897 |
| Vwf | 125 | 164 | 6455 | |
| Notch4 | 92 | 198 | 4188 | |
| Nrp1 | 666 | 106 | 46414 | |
| Fibroblastic reticular | Pdgfra | 184 | 98 | 227 |
| Pdgfrb | 90 | 35 | 345 | |
| Des | 96 | 49 | 161 | |
| Hematopoietic | Cd45 | 40 | 15 | 289 |
| T cell | Cd3 (d,e,g) | 6 | 3 | 168 |
| Cd8 (a,b1) | 8 | 3 | 62 | |
| Cd4 | 13 | 8 | 26 | |
| B cell | Cd19 | 18 | 4 | 183 |
| Cd20 | 17 | 6 | 125 | |
| Dendritic cell | Cd11c | 3 | 2 | 4 |
| Macrophage | Cd11b | 1 | 3 | 7 |
| Tolerogenic profile | Tyr | 346 | 9 | 19 |
| Cd274 | 11612 | 866 | 9798 | |
| Housekeeping genes | Actb | 1,052,300 | 1,561,806 | 1,328,475 |
| Hprt | 3837 | 5214 | 4941 |
Genes and FPKM values highlighted in bold represent previously identified lineage and phenotypic markers associated with the cell types.