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. Author manuscript; available in PMC: 2019 Apr 23.
Published in final edited form as: Biochemistry. 2016 Oct 12;55(42):5917–5926. doi: 10.1021/acs.biochem.6b00826

Fig. 2. Nb:LacY complex formation.

Fig. 2.

(a) SDS-12%PAGE. WT LacY or the double-Trp mutant (ca. 1 μg) were loaded in lanes 1 or 2, respectively, without heating. Given Nbs (3 μg) were loaded in lanes 4 or 5 after heating at 95 °C for 15 min in the presence of 5 mM DTT. Molecular weight markers are given in lane 3. (b) Blue native-12%PAGE. Nbs (8 μg), WT LacY (4 μg) or the double-Trp mutant (4 μg) were loaded in the indicated lanes. WT LacY or the double-Trp mutant were mixed with a given Nb at a ratio of 1:2 prior to the analysis. Δ, the position of the Nb:LacY complexes.