Table 1.

Nb binding with ITC at 25 °C^a

Titrant (Syringe)	Titrand (Cell)	Mel (mM)	Kd (mM)	ΔG [ΔΔG]b	ΔH [ΔΔH]	−TΔS [−TΔΔS]	N
				(kJ/mol)
Nb-9036	Double-Trp mutant	0	59.8 (5.1c)	−41.2 (0.2)	−49.4 (0.3)	8.2 (0.5)	1.0 (0.1)
		10	61.2 (8.4)	−41.2 (0.3)	−58.1 (2.6)	16.5 (2.1)	1.0 (0.1)
				[−0.0ns]	[−8.7±]	[8.3±]
	WT LacY	0	114.4 (6.2)	−39.6 (0.2)	−20.1 (1.0)	−19.5 (1.1)	0.9 (0.1)
		10	39.3 (8.2)	−42.3 (0.5)	−38.1 (0.6)	−4.2 (0.7)	1.0 (0.1)
				[−2.7*]	[−18.0**]	[15.3**]
Nb-9065	Double-Trp mutant	0	150.4 (15.4)	−39.0 (0.3)	−40.7 (0.2)	1.8 (0.0)	1.1 (0.0)
		10	340.7 (45.4)	−36.9 (0.3)	−42.9 (1.3)	5.9 (1.0)	1.0 (0.0)
				[2.1*]	[−2.2ns]	[4.1±]
	WT LacY	0	896.5 (173.6)	−34.8 (0.5)	−12.6 (1.2)	−22.3 (1.5)	1.0 (0.1)
		10	138.6 (23.1)	−39.2 (0.5)	−31.4 (0.8)	−7.9 (1.3)	1.2 (0.1)
				[−4.4*]	[−18.9**]	[14.5*]

^aData are from Fig. 1.

^bDifference in the absence and presence of melibiose;

^cs.e.m., number of tests = 2-5; N, stoichiometry;

^nsnot statistically significant;

^±not quite statistically significant;

^*statistically significant;

^**very statistically significant.