Table 1. Investigations of neurologic signs in lambs in 2016 and 2017 in Scotland and Wales *.

Year	Flock	Case information	Case no.	EM	Tested tissue	Cq	OvPV reads, no. (%)†
2016	I	Progressive neurologic signs including tetraparesis, recumbency, ataxia, and death were observed in several lambs beginning at 2–3 weeks of age in a flock of 200 ewes that was accredited free of maedi-visna virus. The lamb submitted for investigation (S012722-2) was being artificially reared. Additional lambs showed similar clinical signs, but they recovered apart from residual slight paresis in one limb.	S012722–1	–	Cerebrum	NA	0
			S012722-2	+	Cerebrum	29.2	1 (0.00005)
2017	I	Neurologic signs resulting from spinal cord compression by a vertebral abscess.	S014148	–	Cerebrum	NA	NP
					Spinal cord	NA	NP
2017	II	Approximately one third of a group of 60 young pet lambs (mainly orphan lambs or triplet lambs because of insufficient milk to rear 3 lambs) being artificially reared were affected in a flock of 650 ewes. Clinical signs reported were stiff back legs, tremors that became exaggerated on handling, progressing to lateral recumbency, terminal seizures, and death or euthanasia. One lamb with mild clinical signs recovered.	S014175	+	Cerebrum	28.1	NP
					Spinal cord	18.2	1,383 (0.06)
			S014176	+	Cerebrum	26.1	NP
					Spinal cord	27.6	NP
			S014177	+	Cerebrum	27.9	NP
					Spinal cord	19.7	590 (0.02)

*Testing by quantitative reverse transcription PCR for ovine picornavirus and by metagenomics. C_q, PCR cycle quantification; EM, encephalomyelitis; NA, not applicable (C_q value in RT-qPCR >45); NP, not performed; OvPV, ovine picornavirus; +, confirmed nonsuppurative encephalomyelitis and ganglionitis; –, no evidence of nonsuppurative encephalomyelitis and ganglionitis; instead, compressive lesions in the spinal cord.
† Number of OvPV reads in the metagenomics dataset, total (proportion).