Table 4. Routes of infection and delivered doses of Select Agents used to infect guinea pigs and rabbits for tissue collection in study of inactivation of bacterial Tier 1 Select Agents.

Bacteria	Route of infection	Delivered dose, CFU	Animal model	Time of collection	Tissue collected
Bacillus anthracis Ames (spores)	Intradermal	2.7 × 108	Guinea pigs-Hartley	2 h after challenge	Skin
	Inhalational and/or intramuscular, respiratory	4.9 × 106 and 1.0 × 103 respiratory	Guinea pigs-Strain 13	Late-stage disease	Lung, liver, spleen
	Inhalational	1.0 × 107	Rabbits-New Zealand White	24 h after challenge	Lung
Burkholderia pseudomallei 1026b	Intraperitoneal	9.0 × 102	Guinea pigs-Strain 13	Late-stage disease	Lung, liver, spleen
Francisella tularensis Schu S4	Subcutaneous	8.6 × 102	Guinea pigs-Strain 13	Late-stage disease	Lung, liver spleen
Yersina pestis CO92	Subcutaneous	7.0 × 104	Guinea pigs-Strain 13	Late-stage disease	Lung, liver, spleen