Table 7. Growth of Bacillus, Burkholderia, Francisella, and Yersinia species in diluted formaldehyde in study of inactivation of bacterial Tier I Select Agents*.
| Strain | CFU/volume | Growth condition | Time, d | NBF concentration, % | Growth |
|---|---|---|---|---|---|
|
Bacillus anthracis Sterne |
5 (bacilli)/500 mL |
BHI, 37°C |
1 |
0.1 | – |
| 0.01 | + | ||||
| 0.001 |
+ |
||||
|
Burkholderia pseudomallei 82 |
10/500 mL |
LB + 4% glycerol, 37°C |
5 |
0.1 | – |
| 0.01 | – | ||||
| 0.001 |
+ |
||||
|
B. pseudomallei 1026b |
5/500 mL |
LB + 4% glycerol, 37°C |
3 |
0.1 | – |
| 0.01 | + | ||||
| 0.001 |
+ |
||||
|
Francisella tularensis LVS |
1.4 × 105/100 mL |
BHI + 1% IsoVitaleX, 37°C |
4 |
0.1 | – |
| 0.01 | – | ||||
| 0.001 |
+ |
||||
| Yersina pestis Pgm–/pPst– | 10/100 mL | HI + 0.2% xylose, 28°C | 3 | 0.1 | – |
| 0.01 | – | ||||
| 0.001 | + |
*5 CFU B. anthracis Sterne (bacilli), 10 CFU B. pseudomallei 82, 5 CFU B. pseudomallei 1026b, 1.4 × 105 CFU F. tularensis live vaccine strain and 10 CFU Y. pestis Pgm–/pPst– bacteria were inoculated into their respective broths containing 0.1%, 0.01%, and 0.001% NBF and grown at the indicated temperature. Time listed is when broth cultures reached turbidity. + /– indicates presence and/or absence of growth in broth. BHI, brain heart infusion; HI, heart infusion; LB, Lennox broth; LVS, live vaccine strain; NBF, neutral buffered formalin.