Table 1. Replicates of pigs orally exposed to ASFV in liquid or feed based on a sequential adaptive experimental design to determine the infectious dose of ASFV when consumed naturally*.
| Dose ASFV, TCID50 |
Liquid media replicates, no. tested (no. positive) |
|
Plant-based feed replicates, no. tested (no. positive) |
|||||||||||||
| 1 |
2 |
3 |
4 |
5 |
6 |
7 |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|||
| 100 | – | – | – | – | 3 (3) | – | 5 (0) | – | – | – | – | – | – | – | ||
| 101 | – | – | 5 (3) | 5 (1)† | – | – | – | – | – | – | – | – | – | – | ||
| 102 | – | 4 (2) | – | – | 2 (2) | 2 (2) | – | – | – | – | – | – | – | – | ||
| 103 | 5 (5) | 1 (0) | – | – | – | – | – | – | 5 (0) | – | – | – | – | – | ||
| 104 | – | – | – | – | – | 3 (3) | – | 5 (2) | – | – | – | – | – | – | ||
| 105 | – | – | – | – | – | – | – | – | – | 5 (2) | 5 (2)† | – | – | – | ||
| 106 | – | – | – | – | – | – | – | – | – | – | – | 3 (0) | – | 5 (2) | ||
| 107 | – | – | – | – | – | – | – | – | – | – | – | 2 (0) | 3 (2) | – | ||
| 108 | – | – | – | – | – | – | – | – | – | – | – | – | 2 (1) | – | ||
*Data are shown for the 5 infected pigs. In each replicate, 1 negative control pig was present. ASFV, African swine fever virus; TCID50, 50% tissue culture infectious dose; –, no pigs tested. †One pig in each of these replicates died before 5 days postinoculation for causes other than ASF and was eliminated from the data analysis.