Skip to main content
. 2019 Apr 23;14(4):e0215317. doi: 10.1371/journal.pone.0215317

Table 2. The results of mixed ANOVAs for three different measures of differential growth rate of the last instar larvae of Ematurga atomaria.

Effect NomDF Ddf F P
Absolute increments* Sex 1 1271 18.96 <0.0001
Day 1 4135 37.75 <0.0001
Plant 1 1271 4.45 0.035
Sex:day 1 4135 0.013 0.91
Sex:plant 1 1271 4.51 0.033
Allometric*** Sex 1 1271 11.08 0.0009
Day 1 4135 202.58 < .0.0001
Plant 1 1271 80.41 < .0.0001
Sex:day 1 4135 0.51 0.47
Sex:plant 1 1271 2.39 0.12
Relative** Sex 1 1271 0.124 0.72
Day 1 4135 847.11 < .0.0001
Plant 1 1271 68.26 < .0.0001
Sex:day 1 4135 0.31 0.58
Sex:plant 1 1271 1.58 0.21

The mass increments of 2nd, 3rd, 4th and 5th days within the last instar were treated as repeated measurements on particular individuals. Host plant, sex, day and sex*day were included into the model as fixed factors, and brood (offspring of an individual female) and individual larva (nested in brood) as random factors; type III sum of squares. Removing non-significant interactions from the models did not have a qualitative effect on other statistics.

* Absolute increments represent individual 24h mass increments.

** Relative differential growth rates were calculated as [log10 (mass at the 2nd measurement / mass at the 1st measurement)].

*** Allometric differential growth rates were calculated [the cubic root of mass at 2nd measurement–the cubic root of mass at 1st measurement].