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. 2019 Feb 21;4(4):e121582. doi: 10.1172/jci.insight.121582

Figure 5. MLN4924 inhibits mitochondrial functions.

Figure 5

(A–H) MDA-MB-231 cells were treated with indicated concentrations of MLN4924 for 24 and 48 hours, then subjected to determination of real-time OCR (A and B). Basal OCR (C), ATP-linked OCR (D), proton-leak OCR (E), maximal OCR (F), non-mitochondrial OCR (G), and reserve-capacity OCR (H) were calculated from data shown in A and B (mean ± SD, n = 3). (I–K) MDA-MB-231 cells were treated with indicated concentrations of MLN4924 for 24 and 48 hours, then subjected to analysis for ATP production (I), mitochondrial membrane potential (J), and mtDNA copy number (K) (mean ± SD, n = 3). (L) MDA-MB-231 cells were treated with indicated concentrations of MLN4924 for 24 and 48 hours, and mitochondrial ROS generation was determined using the mitochondrial superoxide indicator MitoSOX Red. Fluorescence was measured using a FACS instrument. Similar results were obtained from 3 independent experiments. *P < 0.05, **P < 0.01 by 1-way ANOVA.