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. 2019 Feb 21;4(4):e124522. doi: 10.1172/jci.insight.124522

Figure 1. lncRNA Malat1 expression undergoes distinct alteration in differentially activated macrophages.

Figure 1

(A) Mouse BMDMs were treated with 100 ng/ml LPS for the indicated duration of time. Total RNAs were isolated and levels of Malat1 determined by real-time PCR. n = 4; mean ± SD; *P < 0.05, **P < 0.01 compared with time “0”. (B) Human PBMC-derived macrophages were treated with 100 ng/ml LPS for the indicated duration of time. Levels of Malat1 were determined. n = 4; mean ± SD; **P < 0.01 compared with time “0”. (C) Human THP-1–derived macrophages were treated with 100 ng/ml LPS for the indicated duration of time. Levels of Malat1 were determined. n = 3; mean ± SD; *P < 0.05 compared with time “0”. (D) BMDMs were treated with or without 100 ng/ml LPS for 1 hour. ChIP assay was performed. Levels of p65 binding to the Malat1 promoter were determined by real-time PCR. n = 3; mean ± SD; ***P < 0.001 compared with “–LPS”. (E) BMDMs were treated with 5 ng/ml mouse IL-4 for the indicated duration of time. Levels of Malat1 were determined. n = 4; mean ± SD; **P < 0.01 compared with time “0”. (F and G) BMDMs were treated with or without 100 ng/ml LPS for 6 hours or 5 ng/ml IL-4 for 24 hours. Cell fractionation was performed, and RNAs in the cytoplasmic and nuclear fractions were isolated. Levels of tubulin α1 and Sno-142 (F), and Malat1 (G) in each fraction were determined by real-time PCR. n = 3; mean ± SD; **P < 0.01, ***P < 0.001. Two-tailed Student’s t test was used (A–G) to analyze statistical significance. Representative of 2 to 3 independent experiments.